In vitro germination and viability of pollen grain of coconut accessions

Catrine Regina Feitosa Moura, Caroline de Araújo Machado, Ana da Silva Lédo

Resumo


Storage as a means of maintaining the pollen viability is important for the preservation of the genetic variability, facilitates the exchange of germplasm and greatly contributes to the generation of variability obtained from artificial crosses, increasing the efficiency of breeding programs. The objective of this study was to select different culture media for the in vitro germination of pollen grain of dwarf and tall coconut accessions, as well as to determine the viability of pollen grain at room temperature conditions. For this purpose, Brazil Green Dwarf (BGD) and Brazilian Tall (BRA) pollen grains derived from the Coconut Active Germplasm Bank of Embrapa Coastal Tablelands Sergipe were used. To evaluate the effect of different culture media on the in vitro germination of pollen grains of anão verde do Brasil de Jiqui (AVeBrJ) and gigante do Brasil Praia do Forte (GBrPF)  accessions, they were inoculated on to Petri dishes containing 2 ml of culture media. The pollen viability was assessed by staining with 1% acetic carmine and in vitro germination at 0, 24, 48 and 72 hours. The culture medium of Lora is suitable to assess the in vitro germination of pollen grain of the AVeBrJ and GBrPF accessions. The pollen grain of the AVeBrJ accession showed intermediate viability (66.87%) at room temperature up to 23.14 hours by in vitro germination.  The pollen grain of the GBrPF accession showed high viability, above 70%, at room temperature up to 120 hours by in vitro germination.

Palavras-chave


Cocos nucifera; In vitro culture; Culture media

Referências


ABDELGADIR, H. A.; JOHNSON, S. D.; VAN STADEN, J. Pollen viability, pollen germination and pollen tube growth in the biofuel seed crop Jatropha curcas (Euphorbiaceae). South African Journal of Botany, v. 79, p. 132-139, 2012.

ARMENDARIZ, B. H. C. et al. Pollen fertility and female flower anatomy of micropropagated coconut palms. Revista Fitotécnica Mexicana, v. 29, n. 4, p. 373-378, 2006.

BREWBAKER, J. L.; KWACK, B. H. The essential role of calcium in pollen germination and pollen tube growth. American Journal of Botany, v. 50, n. 9, p. 859-865, 1963.

CRUZ, T. V. et al. Germinação in vitro de grãos de pólen em Passiflora suberosa L. para sua utilização em hibridação interespecífica. Revista Brasileira de Fruticultura, v. 30, n. 4, p. 875-879, 2008.

DANTAS, A. C. de M. et al. Viabilidade do pólen e desenvolvimento do tubo polínico em macieira (Malus ssp.). Revista Brasileira de Fruticultura, v. 27, n. 3, p. 356-359, 2005.

FRANÇA, L. et al. Viability of eggplant pollen. Crop Breeding & Applied Biotechnology, v. 9, n. 4, p. 320-327, 2009.

GANESHAN, S. Viability and fertilizing capacity of onion pollen ( Allium cepa L.) stored in liquid nitrogen (-196° C). Topical Agricultural, v. 63, n. 1, p. 46-48, 1986.

EINHARDT, P. M.; CORREA, E. R.; RASEIRA, M. C. B. Comparação entre métodos para testar a viabilidade de pólen de pessegueiro. Revista Brasileira Fruticultura, v. 28, n. 1, p. 5-7, 2006.

FERREIRA, D. F. SISVAR: a computer statistical analysis system. Ciência e Agrotecnologia, v. 35, n. 6, p. 1039-1042, 2011.

FRANZON, R. C.; RASERA, M. C. B. Germinação in vitro e armazenamento de pólen de Eugenia involucrata DC. (Myrtaceae). Revista Brasileira de Fruticultura, v. 28, n. 1, p. 18-20, 2006.

GANESHAN, S. et al. Cryopreservation of pollen. In: REED, B.M. (Ed.) Plant cryopreservation: a practical guide. New York: Springer, p. 443-447. 2008.

HANNA, W. N. Pollen storage in frostless and conventional frost- forming freezers. Crop Science, v. 34, p. 1681-1682, 1994.

KARUN, A. et al. Cryopreservation of coconut (Cocos nucifera L.) pollen. Journal of Plantation Crops, v. 3, n. 3, p. 568-571, 2006.

KARUN, A.; SAJINI, K. K. Cryopreservation of coconut zygotic embryos and pollen. Kasaragod: Central Plantation Crops Research Institute, 2010. 17 p. (Technical Bulletin, 63).

LIU, L.; HUANG, L.; LI, Y. Influence of boric acid and sucrose on the germination and growth of areca pollen. American Journal of Plant Sciences, v. 4, p. 1669-1674, 2013.

LORA, M. A. J. et al. Low temperature storage and in vitro germination of cherimoya (Annona cherimola Mill) pollen. Scientia Horticulturae, v. 1, n. 1, p. 91-94, 2006.

OLIVEIRA, M. S. P.; COUTURIER, G.; BESERRA, P. Biologia da polinização da palmeira tucumã (Astrocaryum vulgare Mart.) em Belém-Pará, Brasil. Acta Botanica Brasílica, v. 17, n. 3, p. 343-353, 2003.

PIO, L. A. S. et al. Viabilidade do pólen de laranjas doces em diferentes condições de armazenamento. Ciência e Agrotecnologia, v. 31, n. 1, p. 147-153, 2007.

RANASINGHE, C. S. et al. Approach to Screen Coconut Varieties for High Temperature Tolerance by in-vitro Pollen Germination. Cocos, v. 19, n. 1, p. 1–11, 2010.

RAMOS, J. D. et al. Receptividad del estigma y ajuste del protocolo para la germinación in vitro de granos del polen en cítricos. Interciência, v. 33, n. 1, p. 51-55, 2008.

REIS, R. V. dos et al. Viabilidade in vitro de grãos de pólen de bananeira sob diferentes concentrações de ácido bórico e sacarose. Ciência e Agrotecnologia. v. 35, n. 3, p. 547-553, 2011.

SALLES, L. A. et al. Sacarose e pH na germinação in vitro de grãos de pólen de citros. Ciência e Agrotecnologia, v. 30, n. 1, p.170-174, 2006.

SANTOS, G. et al. Manual on standardized research techniques in coconut breeding. Roma: IPGRI, 1996. 45 p.

SOARES, T. L. et al. In vitro germination and viability of pollen grains of banana diploids. Crop Breending and Applied Biotechnology, v. 8, p. 111-118, 2008.

SOUSA, V. A.; SCHEMBERG, E. A.; AGUIAR, A. V. Germinação in vitro do pólen de jerivá (Syagrus romanzoffiana (S.) Cham) Scientia Forestalis, v. 38, n. 86, p. 147-151, 2010.

SOUZA, M. M.; PEREIRA, T. N. S.; MARTINS, E. R. Microsporogênese e microgametogênese associadas ao tamanho do botão floral e da antera e viabilidade polínica em maracujazeiro amarelo (Passiflora edulis Sims f. flavicarpa Degener). Ciência e Agrotecnologia, v. 26, p. 1209-1217, 2002.

STANLEY, R. G.; LINSKENS, H. F. Pollen: biology, biochemistry and management. New York: Springer - Verlag, 1974, 172 p.

TOWILL, L. E.; WALTERS, C. Cryopreservation of pollen. In: ENGELMANN, F.; TAKAGI, H. (Ed.) Cryopreservation of tropical plant germplasm: current research progress and application. Rome: IPGRI, p.115-129, 2000.


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