Indicators of soil quality in the implantation of no-till system with winter crops

Marco Antonio Nogueira, Tiago Santos Telles, Dáfila dos Santos Lima Fagotti, Osmar Rodrigues Brito, Cássio Egídio Cavenaghi Prete, Maria de Fátima Guimarães


We assessed the effect of different winter crops on indicators of soil quality related to C and N cycling and C fractions in a Rhodic Kandiudult under no-till system at implantation, during two growing seasons, in Londrina PR Brazil. The experimental design was randomized blocks with split-plot in time arrangement, with four replications. The parcels were the winter crops: multicropping of cover crops with black oat (Avena strigosa), hairy vetch (Vicia villosa) and fodder radish (Raphanus sativus); sunflower (Heliantus annuus) intercropped with Urochloa ruziziensis; corn (Zea mays) intercropped with Urochloa; and corn; fodder radish; or wheat (Triticum aestivum) as sole crops. The subplots were the years: 2008 and 2009. Determinations consisted of total organic C, labile and resistant C, total N, microbial biomass C and N, the C/N ratio of soil organic matter, and the microbial quotient (qMic), besides microbiological and biochemical attributes, assessed only in 2009. The attributes significantly changed with the winter crops, especially the multicropping of cover crops and fodder radish, as well as effect of years. Despite stimulating the microbiological/biochemical activity, fodder radish cropping decreased the soil C in the second year, likewise the wheat cropping. The multicropping of cover crops in winter is an option for management in the establishment of no-till system, which contributes to increase the concentrations of C and stimulate the soil microbiological/biochemical activity.


Bioindicators; Organic Mater; Carbon; Microbial biomass

Texto completo:



ALEF, K. Soil respiration. In: ALEF, K.; NANNIPIERI, P., Eds. Methods in Applied Soil Microbiology and Biochemistry. London, Academic Press, 1995. p. 214-219.

ANDERSON, T-H. Microbial eco-physiological indicators to assess soil quality. Agriculture, Ecosystems and Environment, v. 98, n. 1-3, p. 285-293, 2003.

ANGHINONI, I. Fertilidade do solo e seu manejo em sistema plantio direto. In: NOVAIS, R. F. et al., Eds. Fertilidade do Solo. Viçosa, Sociedade Brasileira de Ciência doSolo, 2007. p. 873-928.

BLAIR, G. J.; LEFROY, R. D. B.; LISLE, L. Soil carbon fractions based on their degree of oxidation, and the development of a carbon management index for agricultural system. Australian Journal of Agriculture Research, v. 46, n. 7, p. 1459-1466, 1995.

BINI, D. et al. Identifying indicators of C and N cycling in a clayey Ultisol under different tillage and uses in winter. Applied Soil Ecology, v. 76, p. 95-101, 2014.

BINI, D. et al. Effect of land use on soil organic carbon and microbial processes associated with soil health in southern Brazil. European Journal of Soil Biology, v. 55, p. 117-123, 2013.

BREMNER, J. M.; MULVANEY, C. S. Nitrogen-total. In: PAGE, A. L.; MILLER, R. H. and KEENEY, D. R., Eds. Methods of Soil Analysis, part 2: Chemical and Microbiological Properties. Madison, American Society of Agronomy, 1982. p. 595-624.

BROOKES, P. C. et al. Chloroform fumigation and the release of soil nitrogen: a rapid direct extraction method to measure microbial biomass nitrogen in soil. Soil Biology and Biochemistry, v.17, p. 837-842, 1985.

CARDOSO, E. J. B. N. et al. Soil health: looking for suitable indicators. What should be considered to assess the effects of use and management on soil health? Scientia Agricola, v. 70, n. 4, p. 280-295, 2013.

CASIDA JR., L. E.; KLEIN, D. A.; SANTORO, T. Soil dehydrogenase activity. Soil Science, v. 98, p. 371-376, 1964.

CORDEIRO, M. A. S.; CORÁ, J. E.; NAHAS, E. Atributos bioquímicos e químicos do solo rizosférico e não rizosférico de culturas em rotação no sistema de semeadura direta. Revista Brasileira Ciência do Solo, v. 36, p. 1794-1803, 2012.

EMBRAPA - Empresa Brasileira de Pesquisa Agropecuaria.. Manual de análises químicas de solos, plantas e fertilizantes. 2. ed. Brasília: Embrapa Informação Tecnológica, 2009. 627 p.

EMBRAPA - Empresa Brasileira de Pesquisa Agropecuaria. Sistema Brasileiro de Classificação de Solos. 2. ed. Rio de Janeiro: Embrapa Solos, 2006. 306 p.

FAGOTTI, D. S. L. et al. Gradients in N-cycling attributes along forestry and agricultural land-use systems are indicative of soil capacity for N supply. Soil Use and Management, v. 28, p. 292-298, 2012.

FRANCHINI, J. C. et al. Evolution of crop yields in different tillage and cropping systems over two decades in southern Brazil. Field Crops Research, v. 137, p. 178-185, 2012.

FRANK, D. A.; GROFFMAN, P. M. Plant rhizospheric N processes: what we don’t know and why we should care. Ecology, v. 90, n. 6, p. 1512-1519, 2009.

FRANKENBERGER, W. T.; TABATABAI, M. A. Asparaginase activity of soils. Biology and Fertility of Soils, v. 11, p. 6-12, 1991.

GAMA-RODRIGUES, E. F. et al. Atributos químicos e microbianos de solos sob diferentes coberturas vegetais no norte do Estado do Rio de Janeiro. Revista Brasileira de Ciência do Solo, v. 32, n. 4, p. 1521-1530, 2008.

KASCHUK, G.; ALBERTON, O.; HUNGRIA, M. Three decades of soil microbial biomass studies in Brazilian ecosystems: Lessons learned about soil quality and indications for improving sustainability. Soil Biology and Biochemistry, v. 42, n. 1, p. 1-13, 2010.

KEENEY, D. R.; NELSON, D. W. Nitrogen inorganic forms. In: PAGE, A. L.; MILLER, R. H.; KEENEY, D. R., Eds. Methods of Soil Analysis, part 2: Chemical and Microbiological Properties. Madison, American Society of Agronomy, 1982. p. 643-698.

KUWANO, B. H. et al. Soil quality indicators in a Rhodic Kandiudult under different uses in Northern Paraná, Brazil. Revista Brasileira de Ciência do Solo, v. 38, n. 01, p. 50-59, 2014.

NOGUEIRA, M. A. et al. Promising indicators for assessment of agroecosystems alteration among natural, reforested and agricultural land use in southern Brazil. Agriculture, Ecosystems and Environment, v. 115, p. 237-247, 2006.

PAVAN, M. A. et al. Manual de análise química de solo e controle de qualidade. Londrina: IAPAR, 1992. 39 p. (IAPAR. Circular, 76).

RANGEL, O. J. P.; SILVA, C. A. Estoques de carbono e nitrogênio e frações orgânicas de Latossolo submetido a diferentes sistemas de uso e manejo. Revista Brasileira de Ciência do Solo, v. 31, n. 6, p. 1609-1623, 2007.

SCHINNER, F.; von MERSI. W. Xylanase-, CM-cellulase and invertase activity in soil: an improved method. Soil Biology and Biochemistry, v. 22, p. 511-515, 1990.

SCHOMBERG, H. H.; ENDALE, D. M. Cover crop effects on nitrogen mineralization and availability in conservation tillage cotton. Biology and Fertility of Soils, v. 40, n. 6, p. 398-405, 2004.

SCHUSTER, E.; SCHRODER, D. Side effects of sequentially-applied pesticides on target soil microorganisms: field experiments. Soil Biology and Biochemistry, v. 22, p. 367-373, 1990.

SILVA, A. A. et al. Desempenho agronômico e econômico do milho irrigado em sucessão a espécies invernais de cobertura de solo e/ou para produção de grãos. Ciência Rural, v. 38, n. 3, p. 620-627, 2008.

SILVA, M. B. et al. Atributos biológicos do solo sob influência da cobertura vegetal e do sistema de manejo. Pesquisa Agropecuária Brasileira, v.42, n.12, p.1755-1761, 2007.

SPARLING, G. P. Ratio of microbial biomass carbon to soil organic carbon as a sensitive indicator of changes in soil organic matter. Australian Journal of Soil Research, v. 30, n. 2, p. 195-207, 1992.

TABATABAI, M. A.; BREMNER, J. M. Use of p-nitrofenol phosphate for assay of soil phosphatase activity. Soil Biology and Biochemistry, v.1, p. 301-307, 1969.

TABATABAI, M. A.; BREMNER, J. M. Assay of urease activity in soil. Soil Biology and Biochemistry, v. 4, n. 4, p. 479-487, 1972.

VANCE, E. D.; BROOKES, P. C.; JENKINSON, D. S. An extraction method for measuring soil microbial biomass C. Soil Biology and Biochemistry, v. 19, n. 6, p. 703-707, 1987.

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