Biochemical changes in hybrid pumpkin seeds at different stages of maturation

Patricia Pereira da Silva, Antonio Carlos Souza Albuquerque Barros, Denise Cunha Fernandes dos Santos Dias, Marcelo Coelho Sekita, Warley Marcos Nascimento


This study aimed to evaluate biochemical changes in seeds of the pumpkin hybrid, ‘Jabras’, from fruit harvested at different stages of maturation (15, 30, 45, 60 and 75 days after anthesis). Thirty fruit were harvested at each stage, with the seeds from 15 of the fruit being extracted immediately. The remaining 15 were stored for twenty days in plastic boxes and the seeds extracted after this period. After processing and drying the seeds, the following were determined: moisture content, germination, first count and antioxidant enzyme activity (peroxidase, ascorbate peroxidase, catalase and superoxide dismutase). Seeds from the fruit harvested at 30 DAA displayed low values for germination and vigour and high antioxidant enzyme activity, indicating that they were immature and that drying possibly caused damage to the system of cell membranes. The results obtained in this study demonstrated that analysis related to changes in the activity of enzymes during development and maturation of the seeds was effective in evaluating the physiological and biochemical changes in pumpkin seeds of the ‘Jabras’ cultivar.


Cucurbita maxima; Cucurbita moschata; Antioxidant enzymes

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BRASIL. Ministério da Agricultura, Pecuária e Abastecimento. Regras para análise de sementes. Brasília, DF, 2009. 399 p.

DEL LONGO, O.T. et al. Antioxidant defenses under hyperoxygenic and hyperosmotic conditions in leaves of two lines of maize with differential sensitivity to drought. Plant Cell Physiology, v. 34, p. 1023-1028, 1993.

FERREIRA, I. C. F. R; ABREU, R. W. V. Stress oxidative, antioxidante e fitoquímicos. Bioanálise, v. 2, p. 32-39, 2007.

FIGUEIREDO NETO, A. et al. Maturação fisiológica de sementes de abóbora (Curcubita moschata Duch) produzidas no semiárido. Comunicata Scientiae, v. 5, p. 302-310, 2014.

FURIAN, A. F. et al. Methylene blue prevents methylmalonate-induced seizures and oxidative damage in rat striatum. Neurochemistry Internactional, v. 50, p. 164-171, 2007.

GILL, S. S.; TUTEJA, N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry, v. 48, p. 909-930, 2010.

GRATÃO, P. L. et al. Phytoremediation: green technology for the clean up of toxic metals in the environment. Brazilian Journal of Plant Physiology, v. 17, p. 53-64, 2005.

HORVÁTH, E. et al. Exogenous 4-hydroxybenzoic acid and salicylic acid modulate the effect of short-term drought and freezing stress on wheat plants. Plant Science, v. 51, p. 480-487, 2007.

KRANNER, I. et al. What is stress? concepts, definition and applications in seed science. New Phytologist, v. 188, p. 655-673, 2010.

MARTINS, L. L.; MOURATO, M. P. Alterações no metabolismo de plantas em meios contaminados por metais pesados: estresse oxidativo. Revista Agros, v. 8, p. 6, 2008.

MCDONALD, M.B. Seed Deterioration: Physiology repair and assessment. Seed Science and Technology, v. 22, p. 531-539, 1999.

MOLLER, I. M.; JÉNSEN, P. E.; HANSSON, A. Oxidative modifications to cellular components in plants. Annual Review of Plant Biology, v. 58, p. 459-81, 2007.

NAKADA, P. G. et al. Desempenho fisiológico e bioquímico de sementes de pepino nos diferentes estádios de maturação. Revista Brasileira de Sementes, v. 33, p. 113-122, 2011.

NTULI, T. M. et al. Increased drying rate lowers the critical water content for survival in embryonic axes of English oak (Quecus robus L.) seeds. Journal of Integrative Biology, v. 53, p. 270-280, 2011.

OLIVER, M. J.; BEWLEY, J. D. Desiccation tolerance of plant tissues: A mechanistic overview. Horticultural Reviews, v. 18, p. 171-213, 1997.

SALAMA, Z. A.; GAAFAR, A. A.; FOULY, M. M. E. Genotypic variations in phenolic, flavonoids and their antioxidant activities in maize plants treated with Zn (II) HEDTA grown in salinized media. Agricultural Sciences, v. 6, n. 3, p. 30-39, 2015.

SOFO, A. et al. Antioxidant defences in olive trees during drought stress: changes in activity of some antioxidant enzymes, Functional. Plant Biology, v. 32, p. 45-53, 2005.

VIDIGAL, D. S. et al. Alterações fisiológicas e enzimáticas durante a maturação de sementes de pimenta (Capsicum annuum l.). Revista Brasileira de Sementes, v. 31, p.129-136, 2009.

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