Expansion of the biocontrol spectrum of foliar diseases in rice with combinations of rhizobacteria

Ismail Teodoro de Souza Júnior, Jaqueline Tavares Schafer, Bianca Obes Corrêa, Gustavo Daltrozo Funck, Andréa Bittencourt Moura


The cultivation of irrigated rice is subject to the occurrence of various diseases, where damage causes losses in productivity. Alternatives are currently being sought to control these diseases, with biocontrol a viable possibility. With the aim of broadening the spectrum of action of biocontrol, this study evaluated the effect of combinations of biocontrol bacteria on rice blast (Pyricularia grisea), brown spot (Bipolaris oryzae) and leaf scald (Gerlachia oryzae) in rice plants. The effect of isolate combinations was assessed by microbiolization of seeds from the rice cultivar El Paso L144 with suspensions of the bacteria DFs185 (Pseudomonas synxantha), DFs223 (P. fluorescens), DFs306 (unidentified), DFs416 and DFs418 (Bacillus sp.), used individually or in a combination of two, three or four compatible isolates. Seeds treated with Carboxin Thiram fungicide (T+F) at a final dosage corresponding to 3 ml kg-1 were used as control. Considering the leaf diseases together, eight treatments significantly controlled all three diseases, and were superior to the treatment with fungicide. Among these treatments, seven were combinations. Only the DFs306 bacteria were able to control the three diseases when used individually. It can therefore be presumed that combinations of bacteria, when microbiolized in rice seeds, can broaden the spectrum of action for the control of rice blast, brown spot and leaf scald.


Oryza sativa; Pyricularia grisea; Bipolaris oryzae; Gerlachia oryzae; Seed microbiolization

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AHMED, E.; HOLMSTRÖM, S. J. M. Siderophores in environmental research: roles and applications. Microbial Biotechnology, v. 7, n. 3, p. 196-208, 2014.

BEDENDO, I. P.; PRABHU, A. S. Doenças do arroz (Oryza sativa L.). In: KIMATI, H. et al. Manual de fitopatologia: doenças de plantas cultivadas. 3. ed. São Paulo: Agronômica Ceres, 2005. v. 2, p. 85-99.

CANTERI, M. G et al. SASM-Agri: sistema para análise e separação de médias em experimentos agrícolas pelos métodos Scott-Knott, Tukey e Duncan. Revista Brasileira de Agrocomputação, v. 1, n. 2, p. 18-24, 2001.

CHANDLER, S. et al. Role of cyclic lipopeptides produced by Bacillus subtilis in mounting induced immunity in rice (Oryza sativa L.) Physiological and Molecular Plant Pathology, v. 90, n. 1, p. 20-30, 2015.

CHAVES, P. N. et al. Comining endophytic fungi and bactéria for the biocontrol of Radopholus similis (Cobb) Thorne andfor effects on plant growth. Scientia Horticulturae, v. 122, n. 3, p. 472-478, 2009.

CHOUDHARY, D. K.; JOHRI, B. N. Interactions of Bacillus spp. and plants - with special reference to induced systemic resistance (ISR). Microbiological Research, v. 164, n. 5, p. 493-513, 2009.

CORRÊA, B. O.; SCHAFER, J. T.; MOURA A. B. Spectrum of biocontrol bacteria to control leaf, root and vascular diseases of dry bean. Biological Control, v. 72, n. 1, p. 71-75, 2014.

DIMKIC, I. et al. Additive and synergistic effects of Bacillus spp. isolates and essential oils on the control of phytopathogenic and saprophytic fungi from medicinal plants and marigold seeds. Biological Control, v. 87, n. 1, p. 6-13, 2015.

HABE, M. H.; UESUGI, C. H. Método in vitro para avaliar a capacidade colonizadora de bactérias em raízes de tomateiro. Fitopatologia Brasileira, v. 25, n. 4, p. 657-660, 2000.

INTERNATIONAL RICE RESEARCH INSTITUTE. Sistema de evaluación stándart para arroz. Los Baños, 1975. 64 p.

INTERNATIONAL RICE RESEARCH INSTITUTE. Standardad evaluation system for rice. Manilla: INGER, Genetic Resources Center, 1996. 52 p.

KARTHIKEYAN, V.; GNANAMANICKAM, S. S. Biological control of Setaria blast (Magnaporthe grisea) with bacterial strains. Crop Protection, v. 27, n. 2, p. 263–267, 2008.

KHABBAZ, S. E. et al. Characterisation of antagonistic Bacillus and Pseudomonas

strains for biocontrol potential and suppression of damping-off and root rot diseases. Annals of Applied Biology, v. 166, n. 3, p. 456-471, 2015.

LUDWIG, J. et al. Biocontrole da mancha parda e da escaldadura em arroz irrigado, pela microbiolização de sementes. Tropical Plant Pathology, v. 34, n. 5, p. 322-328, 2009.

LUDWIG, J; MOURA, A. B. Controle biológico da queima-das-bainhas em arroz pela microbiolização de sementes com bactérias antagonistas. Fitopatologia Brasileira, v. 32, n. 5, p. 48-53, 2007.

MAFFIA, A. L. Programa para cálculo de área abaixo da curva do progresso da doença (AACPD) GW-BASIC 3.20. Viçosa, MG: UFV, 1986.

MAKETON, M.; APISITSANTIKUL, J.; SIRIRAWEEKUL, C. Greenhouse evaluation of Bacillus subtilis ap-01 and Trichoderma harzianum AP-001 in controlling tobacco diseases. Brazilian Journal of Microbiology, v. 39, n. 2, p. 296-300, 2008.

NUNES, C. D.; RIBEIRO A. S.; TERRES, A. L. Principais doenças do arroz irrigado e seu controle. In: GOMES, A. S.; MAGALHÃES JUNIOR, A. M. Arroz irrigado no sul do Brasil. Embrapa: Informação Tecnológica, 2004. p. 579-633.

OU, S. H. Rice disease. 2. ed. Kew: Commonwealth Mycological Institute, 1985. 308 p.

PAULITZ, T. C.; AHMAD, J. S.; BAKER, R. Integration of Pythium nunn and Trichoderma harzianum isolate T-95 for the biological control of Pythium damping-off of cucumber. Plant Soil, v. 121, n. 2, p. 243-250, 1990.

PIERSON, E. A.; WELLER, D. M. Use of mistures of fluorescent pseudomonads to suppress take-all and improve the growth of wheat. Phytopathology, v. 84, n. 9, p. 940-947, 1994.

PIVIC, R. et al. Bacterial antagonists Bacillus sp. Q3 and Pseudomonas clororaphis Q16 capable to control wheat powdery mildew in wheat. Romanian Biotechnological Letters, v. 20, n. 3, p. 10448-10460, 2015.

PRABHU, A. S.; FILIPPI, M. C. Arroz (Oryza sativa L.) controle de doenças. In: VALE, F. X. R.; ZAMBOLIM, L. Controle de doenças de plantas. Viçosa, MG: Ministério da Agricultura e Abastecimento, 1997. v. 1, p. 554.

RAUPACH, G. S.; KLOEPPER, J. W. Mixtures of plant growth-promoting rhizobacteria enhance biological control of multiple cucumber pathogens. Phytopathology, v. 88, n. 11, p. 1158-1164, 1998.

ROMEIRO, R. S. Controle biológico de enfermidades de plantas: procedimentos. Viçosa, MG: UFV, 2007. 172 p.

SHANMUGAM, V.; KANOUJIA, N. Biological manegement of vascular wilt of tomato caused by Fusarium oxysporum f. sp. lycopersici by plant growth-promoting rhizobacterial mixture. Biological Control, v. 57, n. 2, p. 85-93, 2011.

SIVASAKTHI, S.; USHARANI, G.; SARANRAJ, P. Biocontrol potentiality of plant growth promoting bacteria (PGPR) - Pseudomonas fluorescens and Bacillus subtilis: a review. African Journal of Agricultural, v. 9, n. 16, p. 1265-1277, 2014.

SNEH, B. et al. Chlamydospore germination of Fusarium oxysporum f. sp. cucumerinum as affected by fluorescent and lytic bacteria from a Fusarium-suppressive soil. Phytopathology, v. 74, n. 9, p. 1115-1124, 1984.

SOUZA JÚNIOR, I. T. et al. Biocontrole da queima das bainhas e do nematoide das galhas e promoção de crescimento de plantas de arroz por rizobactérias. Pesquisa Agropecuária Brasileira, v. 45, n. 11, p. 1259-1267, 2010.

VIDHYSEKARAN, P. et al. Induction of systemic resistance by Pseudomonas fluorescens Pf1 against Xanthomonas oryzae pv. oryzae in rice leaves. Phytoparasitica, v. 29, n. 1, p. 155-166, 2001.

WEI, H.; ZHANG, L. Quorum-sensing system influences root colonization and biological control ability in Pseudomonas fluorescens 2P24. Antonie Van Leeuwenhoek, v. 89, n. 2, p. 267-280, 2006.

YU, X. et al. The siderophore-producing bacterium, Bacillus subtilis CAS15, has a biocontrol effect on Fusarium wilt and promotes the growth of pepper. European Journal of Soil Biology, v. 47, n. 2, p. 138-145, 2011.

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