Blood parameters and enzymatic and oxidative activity in the liver of chickens fed with calcium anacardate

Carlos Eduardo Braga Cruz, Ednardo Rodrigues Freitas, Nádia de Melo Braz, Rosa Patrícia Ramos Salles, Isaac Neto Gomes da Silva


The aim of this research was to evaluate the inclusion of calcium anacardate (CAC) as a source of anacardic acid in the diet of broiler chickens on blood parameters, and enzymatic and oxidative activity in the liver. A total of 840 male chicks, one day old, were kept in a completely randomised experimental design, with six treatments and seven replications of 20 birds, totalling 140 birds per treatment. The treatments consisted of feed without the addition of growth promoter (GP), feed with GP, and feed with no GP and the addition of CAC at levels of 0.25, 0.50, 0.75 and 1%. The biochemical blood variables to be analysed were uric acid, total cholesterol, HDL, LDL, creatinine, AST, ALT, triglycerides, total erythrocytes, haemoglobin, haematocrit, mean corpuscular volume, corpuscular haemoglobin concentration, total plasma protein, total leukocytes, heterophils, lymphocytes, platelets and heterophil/lymphocyte ratio. The concentrations of superoxide dismutase, glutathione peroxidase and malondialdehyde were analysed for the enzymatic and oxidative parameters in the liver. There were no significant differences between treatments in the blood parameters or the enzymatic and oxidative activity in the liver of the chickens, demonstrating that the use of calcium anacardate as a source of anacardic acid is non-toxic, and does not affect these parameters.


Additives; Anacardic acid; Haemogram; Leukogram

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ABDEL-FATTAH, S. A. et al. Thyroid activity, some blood constituents, organs morphology and performance of broiler chicks fed supplemental organic acids. International Journal Poultry Science, v. 7, p. 215-222, 2008.

ACHANATH, R. et al. Antimicrobial derivatives of anacardic acid and process for preparing the same. 2010. Disponível em: , Acesso em: 17 jan. 2015.

AGAR, E. et al. The effect of ethanol on lipid peroxidation and glutathione level in the brain stem of rat. Neuroreport, v. 10, p. 1799-1801, 1999.

AL-SAAD, S. et al. Effects of some growth promoters on blood hematology and serum composition of broiler chickens. International Journal of Agricultural Research, v. 9, p. 265-270, 2014.

BEAUCHAMP, C.; FRIDOVICH, I. Superoxide dismutase: improved assay applicable to acrylamide gels. Analytical Biochemistry, v. 44, p. 276-287, 1971.

BIAVATTI, M. W. et al. Preliminary studies of alternative feed additives for broilers: Alternanthera brasiliana extract, propolis extract and linseed oil. Revista Brasileira de Ciência Avícola, v. 5, p. 141-147, 2003.

BORSA, A. et al. Valores hematológicos em frangos de corte de criação industrial. Colloquium Agrarieae, v. 5, p. 25-31, 2009.

BRENES, A.; ROURA, E. Essential oils in poultry nutrition: main effects and modes of action. Animal Feed Science and Technology, v. 158, p. 1-4, 2010.

GROSS, W. B.; SIEGEL, P. B. General principles of stress and welfare. In: GRANDIN, T. (Ed.). Livestock handling and transport. Wallingford, UK : CAB International, p. 21-34, 1993.

HA, T. J.; KUBO, I. Lipoxygenase inhibitory activity of anacardic acids. Journal of Agricultural Food Chemistry, v. 53, p. 4350-4354. 2005.

HAMAD, F. B.; MUBOFU, E. B. Potential biological applications of bio-based anacardic acids and their derivatives. International Journal of Molecular Sciences, v. 16, p. 8569-8590, 2015.

HOSSEINI-VASHAN, S. J. et al. Antioxidant status, immune system, blood metabolites and carcass characteristic of broiler chickens fed turmeric rhizome powder under heat stress. African Journal of Biotechnology, v. 11, p. 16118-16125, 2012.

KAYA, C. A.; TUNCER, S. D. The effects of an organic acid and etheric oils mixture on fattening performance, carcass quality and some blood parameters of broilers. Journal of Animal and Veterinary Advances, v. 8, p. 94-98, 2009.

KONAN, N. A. et al. Acute, subacute toxicity and genotoxic effect of hydroethanolic extract of the cashew (Anacardium occidentale L.) Journal of Ethnopharmacol, v. 110, p. 30 – 38, 2007.

KOYAMA, T. et al. Nutritional control of body size through Foxo-ultraspiracle mediated ecdysone biosynthesis. PubMed, v. 25, n. 3, 2014.

KUBO, I. et al. Antioxidant activity of anacardic acids. Food Chemistry, v. 99, p. 555-562, 2006.

MINAFRA, C. S. et al. Perfil bioquímico do soro de frangos de corte alimentados com dieta suplementada com alfa-amilase de Cryptococcus flavus e Aspergillus niger HM2003. Revista Brasileira de Zootecnia, v. 39, n. 12, p. 2991-2996, 2010.

MORAIS, T. C. et al. Protective effect of anacardic acids from cashew (Anacardium occidentale) on ethanol-induced gastric damage in mice. Chemico-Biological Interactions, v. 9, p. 183-264. 2010.

MURUGESAN, G. R. et al. Phytogenic feed additives as an alternative to antbiotic grow promoters in broiler chckens. Veterinary Science, v. 2, p. 21, 2015.

NOURMOHAMMADI, R. et al. Effect of dietary acidification on some blood parameters and weekly performance of broiler chickens. Journal of Animal and Veterinary Advances, v. 9, p. 3092-3097, 2010.

ÖZEK, K. et al. Effects of dietary herbal essential oil mixture and organic acid preparation on laying traits, gastrointestinal tract characteristics, blood parameters and immune response of laying hens in a hot summer season. Journal of Animal and Feed Sciences, v. 20, p. 575–586, 2011.

ROSTAGNO, H. S. et al. Tabelas brasileiras para aves e suínos composição de alimentos e exigências nutricionais. 3. ed. Viçosa, MG: UFV: DZO, 2011. 186 p.

ROTAVA, R. et al. Bioquímica sanguínea de frangos de corte alimentados com subprodutos da uva. Agrarian, v. 1, n. 1, p. 91-104, 2008.

SAS INSTITUTE. SAS/STAT: user’s Guide. Version 9.2 Cary: SAS Institute, 2009. 7869p.

SCHMIDT, E. M. S. et al. Patologia clínica em aves de produção: uma ferramenta para monitorar a sanidade avícola: revisão. Archives of Veterinary Science, v. 12, n. 3, p. 9-20, 2007.

SEDLAK, J.; LINDSAY, R. H. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with ellman’s reagent. Analytical Biochemistry, v. 25, p. 192-205, 1968.

SIM, A. S. et al. Improved method for plasma malondialdehyde measurement by high-performance liquid chromatography using methyl malondialdehyde as an internal standard. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, v. 785, p. 337-344, 2003.

SOLTAN, M. A. Effect of dietary organic acid supplementation on egg production, egg quality and some blood serum parameters in laying hens.International Journal of Poultry Science, v. 7, p. 613-621, 2008.

TAWFEEK, S. S. et al. The effect of dietary supplementation of some antioxidants on performance, oxidative stress, and blood parameters in broilers under natural summer conditions. Journal World’s Poultry Research, v. 4, p. 10-19, 2014.

TESSARI, E. N. et al. Parâmetros hematológicos de frangos de corte alimentados com ração contendo aflatoxina B1 e fumonisina B1. Ciência Rural, v. 36, n. 3, p. 924-929, 2006.

TREVISAN, M. T. S. B. et al. Characterization of alkyl phenols in cashew (Anacardium occidentale) products and assay of their antioxidant capacity. Food and Chemical Toxicology, v. 44, p. 188-197, 2006.

WANG, P. J. et al. Effects of phenyllactic acid on production performance, egg quality parameters, and blood characteristics in laying hens. Journal of Applied Poultry Research, v. 18, p. 202-209, 2009.

YALCIN, S., ONBASILAR, I.; KOCAOGLU, B. Lactic acid in quail nutrition. Veterinary Journal Ankara University, v. 44, p. 169-181, 1997.

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