Bioaccessibility of the bioactive compounds and antimicrobial activity of aqueous extracts of Physalis angulata L.

Flayanna Gouveia Braga Dias, Maria Jaiana Gomes Ferreira, Larissa Morais Ribeiro da Silva, Rayanne Clécia de Sousa Menezes, Evânia Altina Teixeira de Figueiredo

Resumo


Plant diversity around the world, as well as the chemical variation, involves a large amount of bioactive substances. The use of plant extracts with biological properties appears as a viable and healthy alternative when compared to synthetic substances. In this context, the objective of this study was to evaluate the aqueous extracts of native and cultivated leaves of Physalis angulata L., as well as the influence of the extraction method on the bioaccessibility of bioactive compounds, antioxidant activity and antimicrobial activity. The extracts were obtained from the native and cultivated leaves of P. angulata using three different extraction methods: decoction, maceration and an ultrasound-assisted method. The analysis of variance showed significant differences between the extraction methods and the type of plant material. The extracts obtained by decoction showed the highest levels of phenolic compounds and higher antioxidant potential for both analytical methods (ABTS and DPPH), differing significantly from the other extraction methods. The bioaccessibility indices of the phenolic compounds obtained from the extracts were considered reduced after the simulated gastrointestinal digestion, and consequently, showed low antioxidant potential. The antimicrobial potential of the extracts was observed against the Gram-positive bacteria Staphylococcus aureus and Listeria monocytogenes.


Palavras-chave


Antimicrobial potential; Antioxidant potential; Bioactive compounds

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Referências


BARRIADA-BERNAL, L. G. et al. Flavonoid composition and antioxidant capacity of the edible flowers of Agave durangensis (Agavaceae). CyTA - Journal of Food, v. 12, n. 2, p. 105-114, 2014.

BRANDT, A. L. et al. Inhibition of L. monocytogenes by food antimicrobials applied singly and in combination. Journal Joof Food Science, v. 75, n. 9, p. 57-63, 2010.

BRANEN, J. K.; DAVIDSON, P. M. Enhancementofnisin, lysozyme, and monolaurin antimicrobial activities by ethylenediaminetetraacetic acid and lactoferrin. International Journal of Food Microbiology, v. 90, p. 63-74, 2004.

BRIONES-LABARCA, V. et al. Effects of high hydrostatic pressure (HHP) on bioaccessibility, as well as antioxidant activity, mineral and starch contents in Granny Smith apple. Food Chemistry, v. 128, n. 2, p. 520-529, 2011.

CARNIEL, N. et al. The effects of ultrasound-assisted extraction on polyphenolics compounds obtained from Physalis angulata using response surface approach. Science Food Technology and Food Engineering, v. 40, p. 3-6, 2018.

CLINICAL AND LABORATORY STANDARDS INSTITUTE. Performance standards for antimicrobial susceptibility testing. 27 th ed. Wayne, PA: Clinical and Laboratory Institute, 2017. CLSI supplement M100.

COBALEDA-VELASCO, M. et al. Phenolic profiles and antioxidant properties of Physalis angulata L. as quality indicators. Journal of Pharmacy & Pharmacognosy Research, v. 5, n. 2, p. 114-128, 2017.

CRUZ, C. D. Programa Genes: estatística experimental e matrizes. 1. ed. Viçosa: Editora UFV, 2006. v. 1, 285 p.

DONKOR, A.; ODURO-MENSAH, D.; FIAZORLI, M. Extracts of euphobia hirta linn and P. angulata and their amalgamation demonstrate potency against S. aureus and P. aeruginosa. International Journal of Pharmacy and Pharmaceutical Sciences, v. 8, n. 4, p. 322-326, 2016.

FERREIRA, S. B. et al. Avaliação da atividade antimicrobiana in vitro do extrato hidroalcoolico de Stryphnodendron adstringens (Mart.) Coville sobre isolados ambulatoriais de Staphylococcus aureus. Revista Brasileira de Análise Clínica, v. 42, n. 1, p. 7-31, 2010.

GARBETTA, A. et al. Antioxidant activity induced by main polyphenols present in edible artichoke heads: influence of in vitro gastro-intestinal digestion. Journal of Functional Foods, v. 10, p. 456-464, 2014.

GIL-CHÁVEZ, G. j. et al. Technologies for extraction and production of bioactive compounds to be used as nutraceuticals and food ingredients: an overview. Comprehensive Reviews in Food Science and Food, v. 12, n. 1, p. 5-23, 2013.

GYAWALI, R.; IBRAHIM, S. A. Natural products as antimicrobial agents. Food Control, v. 46, p. 412-429, 2014.

HAYEK, S. A. et al. Antimicrobial natural products. Microbial pathogens and atrategies for combating them. Science, Technology and Education, v. 2, p. 910-921, 2013.

HELAL, A. et al. Bioaccessibility of polyphenols and cinnamaldehyde in cinnamon beverages subjected to in vitro gastropancreatic digestion. Journal of Functional Foods, v. 7, p. 506-516, 2014.

HSEU, C. Y. et al. Inhibitory effects of physalis angulata on tumor metastasis and angioneses. Journal of Ethnopharmacology, v. 135, n. 3, p. 762-771, 2011.

KUSUMANINGTYAS, R. W. et al. Potential of Ciplukan (Physalis angulata L.) as source of functional ingredient. Procedia Chemistry, v. 14, n. 1, p. 367-372, 2015.

LARRAURI, J. A.; RUPÉREZ, P.; SAURACALIXTO, F. Effect of drying temperatura on the stabilitity of polyphenols and antioxidant activityof red grape pomacepeels. Journal of Agricultural and Food Chemistry, v. 45, n. 4, p. 1390-1393, 1997.

MEDINA-MEDRANO, J. R. et al. Phenolic constituents and antioxidant properties of five wild species of Physalis (Solanaceae). Botanical Studies, v. 56, n. 1, p. 1-13, 2015.

PALAFOX-CARLOS, H.; AYALA-ZAVALA, J. F.; GONZÁLEZ-AGUILAR, G. A. The role of dietary fiber in the bioaccessibility and bioavailability of fruit and vegetable antioxidants. Journal of Food Science, v. 76, n. 17, p. 1750-3841, 2011.

RAMÍREZ-MORENO, E. et al. Intestinal bioaccessibility of polyphenols and antioxidant capacity of pulp and seeds of cactus pear. International Journal of Food Sciences and Nutrition, v. 62, p. 839-843, 2011.

RENGIFO-SALGADO, E.; VARGAS-ARANA, G. Physalis angulata L. (Bolsa Mullaca): a review of its traditional uses, chemistry and pharmacology. Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas, v. 12, n. 5, p. 431-445, 2013.

RUFINO, M. S. M. et al. Determinação da atividade antioxidante total em frutas pela captura do radical livre ABTS. Embrapa Agroindústria Tropical, n. 128, p. 1-4, 2007.

SCHNEIDER, A. L. S. et al. Caracterização química e atividade biológica de extratos aquosos de Brunfelsia cuneifolia (Solanaceae). Revista Brasileira de Plantas Medicinais, v. 17, n. 4, p.1103-1111, 2015.

SILVA, C.; GARCIA, V. A. S.; FRANCISCATO, L. M. S. S. Extração assistida por ultrassom de compostos bioativos das cascas de lichia (Litchi Chinensis Sonn.). Revista Ciências Exatas e Naturais, v. 37, n. 3, p. 81-96, 2016.

SIMONETTI, E. et al. Avaliação da atividade antimicrobiana de extratos de Eugenia anomala e Psidium salutare (Myrtaceae) frente à Escherichia coli e Listeria monocytogenes. Revista Ciências Exatas e Naturais, v. 18, n. 1, p. 9-18, 2016.

SUSANTI, R. F. et al. Phenol, flavanoid and antioxidant activity of Physalis angulata leaves extract by subcritical water extraction. Modern Applied Science, v. 9, n. 7, p. 190-198, 2015.

TIWARI, P. et al. Phytochemical screening and extraction: a review. International and Pharmaceutica Science, v. 1, n. 1, p. 98-106, 2011.

XYNOS, N. et al. Development of a green extraction procedure with super/subcritical fluids to produce extracts enriched in oleuropein from olive leaves. The Journal of Supercritical Fluids, v. 67, p. 89-93, 2012.




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