Anatomical, histochemical and physiological changes during maturation of chickpea (Cicer arietinum L.) seeds

Ana Clara Reis Trancoso, Denise Cunha Fernandes dos Santos Dias, Edgard Augusto Toledo Picoli, Rubens Alves da Silva Júnior, Laércio Junio da Silva, Warley Marcos Nascimento


The maturation stage of seeds affects their physiological quality soon after harvest. The aim of this study was to evaluate the anatomical, histochemical, and physiological changes in chickpea ‘BRS Aleppo’ seeds obtained from pods in different maturity stages. Pods were collected in five maturity stages: green, yellow-green, yellow, golden-yellow, and brown. For anatomical and histochemical characterization, sections were stained with toluidine blue, Lugol’s solution, Xylidine-Ponceau (XP), and ruthenium red. For evaluation of physiological changes, seeds were dried to 12% (wet basis) water content. The following tests were performed: seed water content, germination, percentage and speed of seedling emergence, and electrical conductivity. A completely randomized experimental design was used, with four replications. As the chickpea seeds develop, the innermost layers of the seed coat collapse. Accumulation of starch and protein were already observed in the seeds of green pods and pectin was prominent compound of the seed coat sclerids. In general, chickpea seeds from yellow, golden-yellow and brown pods have greater germination and vigor.


Anatomy. Histochemistry. Physiological quality. Pulse. Seed development.

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ARAÚJO, A. V. et al. Time of harvest and storability of Crotalaria juncea L. seeds. Revista Ciência Agronômica, v. 49, n. 1, p. 103-111, 2018.

AVELAR, R. I. S. et al. Production and quality of chickpea seeds in different sowing and harvest periods. Journal of Seed Science, v. 40, n. 2, p. 1–10, 2018.

BARBEDO, C. J.; CENTENO, D. C.; FIGUEIREDO-RIBEIRO, R. C. L. Do recalcitrant seeds really exist?. Hoehnea, v. 40, n. 4, p. 583-593, 2013.

BEWLEY, J. D. et al. Seeds: physiology of development, Germination and Dormancy. 3. ed. New York: Springer, 2013. 392p.

BRASIL. Sistema de Consulta à Legislação - SISLEGIS. Ministério da Agricultura, Pecuária e Abastecimento. Secretaria de Defesa Agropecuária. PORTARIA Nº 111, set. 2012.

BRASIL. MAPA - Ministério da Agricultura, Pecuária e Abastecimento. Secretaria de Defesa Agropecuária. Regras para análise de sementes. ed. 1. Brasília: MAPA/ACS, 2009. 305p.

ESKANDARI, H. Seed quality changes in cowpea (Vigna sinensis) during seed development and maturation. Seed Science and Technology, v. 40, n. 1, p. 108-112, 2012.

Food and Agriculture Organization of the United Nations - FAO. FAOSTAT. Food and agriculture data. 2017. Retrieved from: < http://faostat3.>. Access in April 20, 2017.

GONÇALVES, L. S. A. et al. Seed physiological potential of “dedo-de-moça” pepper in relation to maturation stages and rest periods of the fruits. Horticultura Brasileira. v. 36, p. 486-491, 2018.

INMET. Instituto Nacional de Meteorologia. BDMEP: Banco de dados meteorológicos para ensino e pesquisa. Retrieved from:

JOHANSEN, D. A. Plant microtechnique. New York: McGraw-Hill, 1940. 523p.

KHAJEH-HOSSEINI, M.; REZAZADEH, M. The electrical conductivity of soak-water of chickpea seeds provides a quick test indicative of field emergence. Seed Science and Technology, v. 39, n.3, p. 692–696, 2011.

LIMA CRUZ, J. M. F. et al. Physiology maturity and determination of the harvest time of Vignia unguiculata L. Walp. Journal of Experimental Agriculture International. v. 34, n. 2, p. 1-8, 2019.

MAGUIRE, J. D. Speed of germination and seedling emergence and vigor. Crop Science, v. 2, n. 2, p. 176–177, 1962.

MARCOS-FILHO, J. Fisiologia de sementes de plantas cultivadas. Londrina: ABRATES, 2015.659p.

NASCIMENTO, W. M.; ARTIAGA, O. P.; SUINAGA, F. A. Grão-de-bico. In: NASCIMETO, W. M. Hortaliças leguminosas. 1. ed. Brasília, DF: Embrapa Hortaliças, 2016. cap. 3, p. 89–118.

O’BRIEN, T. P.; FEDER, N.; MCCULLY, M. E. Polychromatic staining of plant cell walls by toluidine blue O. Protoplasma, v. 59, n. 2, p. 368–373, 1964.

R CORE TEAM. R Development Core Team. R: A Language and Environment for Statistical Computing, 2019.

SAMARAH, N. H.; ABU-YAHYA, A. Effect of maturity stages of winter- and spring-sown chickpea (Cicer arietinum L.) on germination and vigour of the harvested seeds. Seed Science and Technology, v. 36, n. 1, p. 177–190, 2008.

SHAHEB, M. et al. Effect of harvest times on the yield and seed quality of French bean. SAARC Journal of Agriculture, v. 13, n. 1, p. 1-13, 2015.

SILVA, C. D. et al. Fruit maturation stage on the physiological quality of maroon cucumber seeds. Pesquisa Agropecuária Tropical, v. 49, e53188, 2019

SILVA, L. J. et al. Physiological quality of Jatropha curcas L. seeds harvested at different development stages. Seed Science and Technology, v. 39, p. 572-580, 2011.

SMÝKAL, P. et al. The role of the testa during development and in establishment dormancy of the legume seed. Frontiers in plant science, v. 5, p. 351, 2014.

VIDAL, B. D. C. Acid glycosaminoglycans and endochondral ossification: microspectrophotometric evaluation and macromolecular orientation. Cellular and Molecular Biology, v. 22, p. 45–64, 1977.

VIDIGAL, D. S. et al. Changes in seed quality during fruit maturation of sweet pepper. Scientia Agricola, v. 68, n. 5, p. 535-539, 2011.

VIEIRA, R. D.; KRZYZANOWSKI, F. C. Teste de condutividade elétrica. In: KRZYZANOWSKI, F. C.; VIEIRA, R. D.; FRANÇA NETO, J. Vigor de sementes: conceitos e testes. Londrina: ABRATES, 1999. cap. 4, p. 1–26.

WOOD, J. A.; KNIGHTS, E. J.; CHOCT, M. Morphology of chickpea seeds (Cicer arietinum L.): comparison of desi and kabuli types. Journal of Plant Science, v. 172, n. 5, p. 632–643, 2011.

YANG, X. et al. More than just a coating: Ecological importance, taxonomic occurrence and phylogenetic relationships of seed coat mucilage. Perspectives in Plant Ecology, Evolution and Systematics, v. 14, n. 6, p. 434-442, 2012.

ZHAO, M. et al. Mobilization and role of starch, protein, and fat reserves during seed germination of six wild grassland species. Plant Science, v. 9, n. 234, p. 1-11, 2018.

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