Phenolic compounds, antioxidant and antibacterial activity of extract from leaves and bark of Stryphnodendron adstringens
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ABSTRACT - There is currently growing interest in the investigation of bioactive compounds from natural sources. In this context, this study investigated the phenolic content, antioxidant capacity, and antimicrobial activity of the hydroalcoholic extract (EHA) of the stem barks and leaves of Stryphnodendron adstringens. Plant parts were extracted with ethanol:water (1:1) and filtered in a vacuum to obtain the crude extract. Phenolic content was determined by the Folin–Ciocalteu method. Antioxidant activity was measured using the DPPH test. The antimicrobial activity of extracts was evaluated against Gram-positive cocci: Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis (strain isolated from a patient); and Gram-negative bacilli: Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853). The zone of inhibition and the minimum inhibitory concentration (MIC) of the plant extracts were evaluated by the agar diffusion disc method and broth microdilution, respectively. Total phenolic content of the EHAs was higher in stem barks (970.4 mg GAE/g) than in leaves (693.8 mg GAE/g). In the DPPH assay, all extracts showed high antioxidant activity, above 75% at the concentration of 100 μg/mL. About antimicrobial activity, all the EHAs showed effects against Gram-positive bacteria S. aureus and S. epidermidis, whose MIC ranged from 7.81 μg/mL to 62.5 μg/mL. This finding is important since these pathogens are generally resistant to a variety of widely known antibiotics. Therefore, these results are promising and indicate the use of S. adstringens as a natural antioxidant in foods, medicines and also point to its use in antimicrobial therapy.
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ALAM, M. N.; BRISTI, N. J.; RAFIQUZZAMAN, M. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharmaceutical Journal, v. 21, n. 2, p. 143–152, 2013.
AWOUAFACK, M. D. et al. Antimicrobial activity and cytotoxicity of the ethanol extract, fractions and eight compounds isolated from Eriosema robustum (Fabaceae). BMC Complementary and Alternative Medicine, v. 13, 2013.
BALDIVIA, S. et al. Evaluation of in vitro antioxidant and anticancer properties of the aqueous extract from the stem bark of stryphnodendron adstringens. International Journal of Molecular Sciences, v. 19, n. 8, p. 1–23, 2018.
CHAKRABORTY, S. et al. Antimicrobial activity of Cannabis sativa, Thuja orientalis and Psidium guajava leaf extracts against methicillin-resistant Staphylococcus aureus. Journal of integrative medicine, v. 16, n. 5, p. 350–357, 2018.
CHOI, U.; LEE, C. R. Antimicrobial agents that inhibit the outer membrane assembly machines of gram-negative bacteria. Journal of Microbiology and Biotechnology, v. 29, n. 1, p. 1–10, 2019.
CHOUNA, J. R. et al. Antibacterial endiandric acid derivatives from Beilschmiedia anacardioides. Phytochemistry, v. 70, no. 5, p. 684–688, 2009.
CLSI. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial disk susceptibility testing; Approved Standard. ed. 28th editi, 2018.
CVETANOVIĆ, A. et al. Isolation of apigenin from subcritical water extracts: Optimization of the process. Journal of Supercritical Fluids, v. 120, p. 32–42, 2017.
DZOYEM, J. P.; MCGAW, L. J.; ELOFF, J. N. In vitro antibacterial, antioxidant and cytotoxic activity of acetone leaf extracts of nine under-investigated Fabaceae tree species leads to potentially useful extracts in animal health and productivity. BMC Complementary and Alternative Medicine, v. 14, n. 1, p. 1–7, 2014.
ERDEM, S. A. et al. Blessings in disguise: A review of phytochemical composition and antimicrobial activity of plants belonging to the genus Eryngium. DARU, Journal of Pharmaceutical Sciences, v. 23, n. 1, 2015.
FERREIRA, R. B. R. et al. Osmotic stress induces biofilm production by Staphylococcus epidermidis isolates from neonates. Diagnostic Microbiology and Infectious Disease, v. 94, n. 4, p. 337–341, 2019.
GALIÉ, S. et al. Biofilms in the food industry: Health aspects and control methods. Frontiers in Microbiology, v. 9, n. MAY, p. 1–18, 2018.
GIAOURIS, E. et al. Attachment and biofilm formation by foodborne bacteria in meat processing environments: Causes, implications, role of bacterial interactions and control by alternative novel methods. Meat Science, v. 97, n. 3, p. 298–309, 2014.
GOBBO-NETO, L.; LOPES, N. P. Medicinal plants: Factors influencing the content of secondary metabolites. Quimica Nova, v. 30, n. 2, p. 374–381, 2007.
HASHEMPOUR-BALTORK F. et al. Drug resistance and the prevention strategies in food borne bacteria: An update reviewNo Title. Advanced Pharmaceutical Bulletin, v. 9, p. 335–347, 2019.
HICKL, J. et al. Mediterranean herb extracts inhibit microbial growth of representative oral microorganisms and biofilm formation of Streptococcus mutans. PLoS ONE, v. 13, n. 12, p. 1–24, 2018.
KUETE, V. Potential of Cameroonian plants and derived products against microbial infections: A review. Planta Medica, v. 76, n. 14, p. 1479–1491, 2010.
LOPES-LUTZ, D. et al. Screening of chemical composition, antimicrobial and antioxidant activities of Artemisia essential oils. Phytochemistry, v. 69, n. 8, p. 1732–1738, 2008.
PINHO L, et al. Antimicrobial activity of hydroalcoholic extracts from rosemary, peppertree, barbatimão and erva baleeira leaves and from pequi peel meal. Ciencia Rural, v. 42, p. 326–331, 2012.
RAHMAN, M. M. et al. Total synthesis of acylphloroglucinols and their antibacterial activities against clinical isolates of multi-drug resistant (MDR) and methicillin-resistant strains of Staphylococcus aureus. European Journal of Medicinal Chemistry, v. 155, p. 255–262, 2018.
RICARDO, L. M. et al. Evidence of traditionality of Brazilian medicinal plants: The case studies of Stryphnodendron adstringens (Mart.) Coville (barbatimão) barks and Copaifera spp. (copaíba) oleoresin in wound healing. Journal of Ethnopharmacology, v. 219, p. 319–336, 2018.
SADIQ, M. B. et al. In vitro antioxidant and antimalarial activities of leaves, pods and bark extracts of Acacia nilotica (L.) Del. BMC Complementary and Alternative Medicine, v. 17, n. 1, p. 1–8, 2017.
SALAM, A. M.; QUAVE, C. L. Opportunities for plant natural products in infection control. Current Opinion in Microbiology, v. 45, p. 189–194, 2018.
SCHELIN, J.; SUSILO, Y. B.; JOHLER, S. Expression of staphylococcal enterotoxins under stress encountered during food production and preservation. Toxins, v. 9, o. 12, 2017.
SINGLETON, V. L. ROSSI, J. A. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American journal of Enology and Viticulture, v. 16 (3), p. 144–158, 1965.
SOUSA, C. M. D. M. et al. Fenóis totais e atividade antioxidante de cinco plantas medicinais. Quimica Nova, v. 30, no. 2, p. 351–355, 2007.
SOUZA-MOREIRA, T. M.; QUEIROZ-FERNANDES, G. M.; PIETRO, R. C. L. R. Stryphnodendron species known as “barbatimão”: A comprehensive report. Molecules, v. 23, n. 4, p. 1–25, 2018.
TAKÓ, M. et al. Plant phenolics and phenolic-enriched extracts as antimicrobial agents against. Antioxidants, v. 9, n. 2, p. 1–21, 2020. .
VERGARA, A. et al. Biofilm Formation and Its Relationship with the Molecular Characteristics of Food-Related Methicillin-Resistant Staphylococcus aureus (MRSA). Journal of Food Science, v. 82, n. 10, p. 2364–2370, 2017.
WAMBA, B. E. N. et al. Antistaphylococcal and antibiotic resistance modulatory activities of thirteen cameroonian edible plants against resistant phenotypes. International Journal of Microbiology, v. 2018, 2018.
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