Pretreatment of seeds with plant regulators attenuates salt stress in pumpkin: effects on germination and initial seedling development

Keylan Silva Guirra, Salvador Barros Torres, José Eduardo Santos Barboza da Silva, Moadir Sousa Leite, Francisco Assis Nogueira Neto, Anna Letícia Barbosa Rego, Bruno Silva Guirra, Emanoela Pereira Paiva

Resumo


Pretreatment of seeds results in faster emergence of seedlings and uniform stand, especially under stress. Thus, the objective was to evaluate the action of plant regulators as stress attenuators during germination and initial development of pumpkin seedlings, cv. ‘Baiana Tropical’, irrigated with saline water. For this, a completely randomized design was established, in a 4 x 3 factorial scheme, with four replications. Treatments consisted of four types of water (W1 - 100% supply water; W2 - 100% fish farming effluent; W3 - 100% artesian well water; W4 - mixture of 50% fish farming effluent + 50% artesian well water) and three seed treatments (control, salicylic acid and gibberellic acid). The variables measured were germination, first germination count, shoot and root length, shoot and root dry mass, total soluble sugars, total amino acids and proline. The data were subjected to analysis of variance and Scott-Knott test. Pumpkin seedlings performed osmotic adjustment under saline conditions of W4 water. In addition, the treatment of pumpkin seeds with plant regulators (gibberellic and salicylic acids) favors the germination and initial development of seedlings under conditions of salt stress.

Palavras-chave


Cucurbita moschata. Cucurbitaceae. Gibberellic acid. Salicylic acid. Stress mitigation.

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Referências


ATLASBIG. Mapas e estatísticas do mundo e regiões, 2020. Acessado em: 21/02/2021. Disponível em: https://www.atlasbig.com/pt-br

AMARO, H. T. R. et al. Tratamento de sementes com produtos à base de fertilizantes e reguladores de crescimento. Pesquisa Agropecuária Gaúcha, v.26, n.1, p.222-242, 2020.

ANAYA, F. et al. Influence of salicylic acid on seed germination of Vicia faba L. under salt stress. Journal of the Saudi Society of Agricultural Sciences, v.17, n.1, p.1-8, 2018. https://doi.org/10.1016/j.jssas.2015.10.002

BATES, L. S.; WALDREN, R. P.; TEARE, I. D. Rapid determination of free proline for water-stress studies. Plant and Soil, v.39, p.205-207, 1973.

BEZERRA, R. U. et al. Produção e qualidade da abóbora maranhão sob influência de lâminas de irrigação e doses de nitrogênio. Irriga, v.25, n.1, p.87-101, 2020. http://dx.doi.org/10.15809/irriga.2020v25n1p87-101

BRASIL. Ministério da Agricultura, Pecuária e do Abastecimento. Regras para análise de sementes. Brasília: MAPA, 2009. 395p.

CHUNTHABUREE, S. et al. Alleviation of salt stress in seedlings of black glutinous rice by seed priming with spermidine and gibberellic acid. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, v.42, n.2, p.405-413, 2014. http://dx.doi.org/10.1583/nbha4229688

DOURADO, D. et al. Efeito de bioestimulante em sementes de cedro-rosa. Brazilian Journal of Development, v.6, n.5, p.30306-30319, 2020. http://dx.doi.org/10.34117/bjdv6n5-474

EMATER-GO - Agência Goiana de Assistência Técnica, Extensão Rural e Pesquisa Agropecuária. Produção de abóbora em Rio Verde se torna referência nacional. Comunicação, 2019. Disponível em: https://www.emater.go.gov.br/wp/producao-de-abobora-em-rio-verde-se-torna-referencia-nacional. Acessado em: 17/03/2021.

FERREIRA, D. F. Sisvar: a computer statistical analysis system. Ciência e Agrotecnologia, v.35, p.1039-1042, 2011.

GASTL FILHO, J. et al. Ácido salicílico e potencial germinativo na germinação de sementes de pepino. Revista Inova Ciência & Tecnologia, v. 3, n. 2, p.7-12, 2017.

GUIRRA, K. S. et al. Phytohormones on the germination and initial growth of pumpkin seedlings under different types of water. Revista Brasileira de Engenharia Agrícola e Ambiental, v. 24, n. 12, p. 827-833, 2020. https://doi.org/10.1590/1807-1929/agriambi.v24n12p827-833

GROHS, M. et al. Attenuation of low-temperature stress in rice seedlings. Pesquisa Agropecuária Tropical, v.46, n.2, p.197-205, 2016. http://dx.doi.org/10.1590/1983-40632016v4640436

JINI, D.; JOSEPH, B. Physiological mechanism of salicylic acid for alleviation of salt stress in rice. Rice Science, v.24, n.2, p.97-108, 2017. http://dx.doi.org/10.1016/j.rsci.2016.07.007

KHAN, M. I. R. et al. Salicylic acid-induced abiotic stress tolerance and underlying mechanisms in plants. Frontiers in Plant Science, v.6, p.17-23, 2015. https://doi.org/10.3389/fpls.2015.00462

LEITE, T. S. et al. The use of saline aquaculture effluent for production of Enterolobium contortisiliquum seedlings. Environmental Science and Pollution Research, v. 24, n. 23, p. 19306-19312, 2017. https://doi.org/10.1007/s11356-017-9577-1

LOPES, A. C. et al. Água salina e substratos no crescimento inicial do meloeiro. Irriga, v. 22, n. 3, p. 469-484, 2017.

MATIAS, J. R. et al. Physiological changes in osmo and hydroprimed cucumber seeds germinated in biosaline water. Journal Seed Science, v. 37, n. 1, p. 07-15, 2015.

MIMOUNI, H. et al. Does salicylic acid (SA) improve tolerance to salt stress in plants? A study of SA effects on tomato plant growth, water dynamics, photosynthesis, and biochemical parameters. Omics: a journal of integrative biology, v. 20, n. 3, p. 180-190, 2016. https://doi.org/10.1089/omi.2015.0161

NÓBREGA, J. S. et al. Emergência e crescimento inicial de melancia submetida a salinidade e doses de ácido salicílico. DESAFIOS - Revista Interdisciplinar da Universidade Federal do Tocantins, v. 7, n. 2, p. 162-171, 2020. http://dx.doi.org/10.20873/ufv7-8169

OLIVEIRA, F. de A. et al. Desenvolvimento inicial de cultivares de abóboras e morangas submetidas ao estresse salino. Revista Agro@mbiente On-line, v. 8, p. 222-229, 2014. http://dx.doi.org/10.18227/1982-8470ragro.v8i2.1540

OLIVEIRA, F. A. et al. Uso de bioestimulante como agente amenizador do estresse salino na cultura do milho pipoca. Revista Ciência Agronômica, v. 47, n. 2, p. 307-315, 2016. http://dx.doi.org/10.5935/1806-6690.20160036.

OLIVEIRA, L. L. P. et al. Tolerância de cultivares de algodão (Gossypium hirsutum) à salinidade da água de irrigação. Agropecuária Científica no Semiárido, v. 10, n. 2, p. 66-71, 2014b.

PAIVA, E. P. et al. Germination and biochemical components of Salvia hispanica L. seeds at different salinity levels and temperatures. Acta Scientiarum. Agronomy, v.40, 2018. http://dx.doi.org/10.4025/actasciagron.v40i1.39396

RAFIQUE, N. et al. Pre-sowing A4lication of ascorbic acid and salicylic acid to seed of pumpkin and seedling response to salt. Pakistan Journal of Botany, v. 43, p. 2677-2682, 2011.

SILVA, T. C. F. S. et al. Germinação de sementes de melancia sob diferentes métodos de tratamento com reguladores vegetais. Scientia Plena, v. 10, n. 3, p. 1-15, 2014.

SIMÕES, W. L. et al. Beet cultivation with saline effluent from fish farming. Revista Brasileira de Engenharia Agrícola e Ambiental, v. 20, n. 1, p. 62–66, 2016.

TAIZ, L. et al. Fisiologia e desenvolvimento vegetal. Porto Alegre: Artmed Editora, p.888, 2017.

YEMM, E. W.; COCKING, E. C. The determination of amino-acids with ninhydrin. Analyst, v. 80, p. 209-213, 1955.

YEMM, E. W.; WILLIS, A. J. The estimation of carbohydrates in plant extracts by anthrone. Biochemical Journal, v. 57, p. 508, 1954.




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