Biocompatibility and biodegradation analysis of Nile Tilapia gelatin and apatite membranes
Resumo
Fish farming by-products could represent large-scale raw materials for xenogeneic implants that could be used for bone regeneration. The objective of this research was to analyze the biocompatibility and biodegradation of gelatin (G) and apatite (A) membranes from Nile tilapia. Adult male Swiss mice underwent subcutaneous implantation of biocomposites derived from skins and scales in different concentrations: 60%G:40%A (G1), 70%G:30%A (G2) and 80%G:20%A (G3). Commercial collagen membrane (C+) or implantless surgery (C–) were used as controls. Euthanasia was performed at 1, 3 or 9 weeks for histological analysis of the inflammatory and repair criteria as well as the integrity of each material. The statistical analysis of non-parametric data was performed using the Kruskal-Wallis test and post-hoc Dunn test, with p < 0.05. In vivo results during the experimental period demonstrated progressive improvement in biocompatibility, with G1 and G2 being slightly irritating and G3 non-irritating, just like C+. There were significant differences between test and control groups regarding the presence of neutrophils, lymphocytes, macrophages, foreign body giant cells, neovascularization and connective tissue. There was also a decrease in the integrity of the implants, where G1 maintained greater stability than G3 and G2, but less than C+. All biocomposites proved to be biocompatible and partially biodegradable. G1 suggests greater potential for use as an osteopromoting membrane, with its biological performance associated with higher mineral concentration compared to the organic phase. Future long-term orthotopic studies will be conducted to investigate its osteopromoting action.
Palavras-chave
Texto completo:
PDF (English)Referências
AL-MAAWI, S. et al. In vivo cellular reactions to different biomaterials-Physiological and pathological aspects and their consequences. Seminars in Immunology, v. 29, n. 1, p. 49-61, 2017.
AL-MAAWI, S. et al. In vivo implantation of a bovine-derived collagen membrane leads to changes in the physiological cellular pattern of wound healing by the induction of multinucleated giant cells: an adverse reaction? Frontiers in Bioengineeering and Biotechnology, v. 6, n. 104, p. 1-13, 2018.
ALVES, A. P. N. N. et al. Study of tensiometric properties, microbiological and collagen content in nile tilapia skin submitted to diferente sterilization methods. Cell and Tissue Banking, v. 19, n. 1, p. 373-382, 2018.
ARAÚJO, L. K. et al. Membranas osteopromotoras em odontologia: tendências científicas e análise do mercado brasileiro. In: FADEL, C. B.; MARTINS, A. S.; PINHEIRO, J. C. Odontologia: pesquisa e práticas contemporâneas. 1. ed. São Paulo: Editora Científica, 2021. cap. 9, p. 103-120.
ARAÚJO, L. K. et al. Brazilian dentists’ perceptions of using bone grafts: an inland survey. Acta Odontológica Latinoamericana, v. 33, n. 3, p. 165-173, 2020.
ASGHARI, F. et al. Biodegradable and biocompatible polymers for tissue engineering application: a review. Artificial Cells, Nanomedicine, and Biotechnology, v. 45, n. 2, p. 185-192, 2017.
CALDATO, K.; NAVES, F. K. S.; ZATTA, L. Gelatina extraída de escamas da tilápia do Nilo (Oreochromis niloticus) produzidas no município de Pato Branco: caracterizações e comparações com amostras comerciais. Revista Brasileira de Tecnologia Agroindustrial, v. 13, n. 1, p. 2730-2751, 2019.
CASTRO-SILVA, I. I. et al. Biotechnological potential of by-products of the Brazilian animal protein industry in the generation of xenogeneic biomaterials for bone regeneration. Trends in Research, v. 1, n. 3, p. 1-2, 2018.
DANIELETTO-ZANNA, C. F. et al. Osteopromotion capacity of bovine cortical membranes in critical defects of rat calvaria: histological and immunohistochemical analysis. International Journal of Biomaterials, v. 2020, n. 1, p. 1-9, 2020.
FRANZ, S. et al. Immune responses to implants: a review of the implications for the design of immunomodulatory biomaterials. Biomaterials, v. 32, n. 28, p. 6692-6709, 2011.
HARRIS, J. J.; LU, S.; GABRIELE, P. Commercial challenges in developing biomaterials for medical device development. Polymer International, v. 67, n. 1, p. 969-974, 2018.
HERRERA-VIZCAÍNO, C. et al. Modification of collagen-based sponges can induce an upshift of the early inflammatory response and a chronic inflammatory reaction led by M1 macrophages: an in vivo study. Clinical Oral Investigation, v. 24, n. 10, p. 3485-3500, 2020.
JACOB, J. et al. Piezoelectric smart biomaterials for bone and cartilage tissue engineering. Inflammation and Regeneration, v. 38, n. 2, p. 2-11, 2018.
JARDELINO, C. et al. Biocompatibility analysis of a novel reabsorbable alloplastic membrane composed of alginate-capsul. Revista Gaúcha de Odontologia, v. 60, n. 4, p. 419-423, 2012.
JARDELINO, C. et al. Porcine peritoneum as source of biocompatible collagen in mice. Acta Cirúrgica Brasileira, v. 25, n. 4, p. 332-336, 2010.
JEONG, J. et al. Bioactive calcium phosphate materials and applications in bone regeneration. Biomaterials Research, v. 23, n. 4, p. 1-11, 2019.
JUNG, S. et al. Effect of gellan gum/tuna skin film in guided bone regeneration in artificial bone defect in rabbit calvaria. Materials, v. 13, n. 6, p. 1-9, 2020.
KONGSRI, S. et al. Nanocrystalline hydroxyapatite from fish scale waste: Preparation, characterization and application for selenium adsorption in aqueous solution. Chemical Engineering Journal, v. 215–216, p. 522–532, 2013.
MARTINS, M. E. O. et al. Thermal and chemical properties of gelatin from tilapia (Oreochromis niloticus) scale. Journal of Aquatic Food Product Technology, v. 27, n. 10, p. 1120-1133, 2018.
MUÑOZ, L. C.; CARDONA-RAMIREZ, S.; SILVA, R. F. Comparison of subcutaneous inflammatory response to commercial and engineered zinc hydroxyapatite implants in rabbits. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 71, n. 6, p. 1873-1879, 2019.
OGAWA, Y. et al. Preparation and biocompatibility of a chitin nanofiber/gelatin composite film. International Journal of Biological Macromolecules, v. 104, n. 1, p. 1882-1889, 2017.
OUYANG, Q. Q. et al. Chitosan hydrogel in combination with marine peptides from tilapia for burns healing. International Journal of Biological Macromolecules, v. 112, n. 1, p. 1191-1198, 2018.
PEREIRA, L. C. et al. In vitro physico-chemical characterization and standardized in vivo evaluation of biocompatibility of a new synthetic membrane for guided bone regeneration. Materials, v. 12, n. 1186, p. 1-12, 2019.
SALGADO, C. L.; TEIXEIRA, B. I. B.; MONTEIRO, F. J. M. Biomimetic composite scaffold with phosphoserine signaling for bone tissue engineering application. Frontiers in Bioengineeering and Biotechnology, v. 7, n. 206, p. 1-16, 2019.
SANCILIO, S. et al. Alginate/hydroxyapatite-based nanocomposite scaffolds for bone tissue engineering improve dental pulp biomineralization and differentiation. Stem Cells International, v. 2018, n. 1, p. 1-13, 2018.
SBRICOLI, L. et al. Selection of collagen membranes for bone regeneration: A literature review. Materials, v. 13, n. 3, p. 1-16, 2020.
SENA, L. A. et al. Biocompatibility of wollastonite-poly(N-butyl-2-cyanoacrylate) composites. Journal of Biomedical Material Research B: Applied Biomaterials, v. 102, n. 6, p. 1121-1129, 2014.
SOUSA-FILHO, M. S. M. et al. Obtenção e caracterização de gelatina de pele de tilápia-do-nilo (Oreochromis niloticus) em escala piloto. 1. ed. Fortaleza: Embrapa Agroindústria Tropical, 2017. 19p.
SOUZA, F. F. P. et al. Poultry by-products as source of collagen, nanokeratin and bioapatite for biomedical use. Revista Ciência Agronômica, v. 52, n. 4, e20207565, 2021.
WANG, J. et al. Feather keratin hydrogel for wound repair: preparation, healing effect and biocompatibility evaluation. Colloids and Surfaces B: Biointerfaces, v. 149, n. 1, p. 341-350, 2017a.
WANG, J. K. et al. Fish scale-derived collagen patch promotes growth of blood and lymphatic vessels in vivo. Acta Biomaterialia, v. 63, n. 1, p. 246-260, 2017b.
WILLIAMS, D. F. The Williams Dictionary of Biomaterials. 1. ed. Liverpool: Liverpool University Press, 1999. 42 p.
Revista Ciência Agronômica ISSN 1806-6690 (online) 0045-6888 (impresso), Site: www.ccarevista.ufc.br, e-mail: ccarev@ufc.br - Fone: (85) 3366.9702 - Expediente: 2ª a 6ª feira - de 7 às 17h.